Nomenclature
Short Name:
MAST2
Full Name:
Microtubule-associated serine-threonine-protein kinase 2
Alias:
- EC 2.7.11.1
- KIAA0807
- MAST205
- Microtubule associated serine/threonine kinase 2
Classification
Type:
Protein-serine/threonine kinase
Group:
AGC
Family:
MAST
SubFamily:
NA
Structure
Mol. Mass (Da):
196,436
# Amino Acids:
1798
# mRNA Isoforms:
2
mRNA Isoforms:
196,436 Da (1798 AA; Q6P0Q8); 176,132 Da (1608 AA; Q6P0Q8-2)
4D Structure:
Interacts with CDHR2.
1D Structure:
Subfamily Alignment
Domain Distribution:
Post-translation Modifications
For detailed information on phosphorylation of this kinase go to PhosphoNET
Acetylated:
K1203, K1207, K1209.
Methylated:
R1004.
Serine phosphorylated:
S66, S74, S81, S92, S144, S148, S151, S160, S183, S191, S204, S209, S230, S278, S281, S290, S297, S299, S301, S305, S806, S825, S846, S854, S855, S860, S874, S876, S883, S895, S900, S914, S918, S920, S931, S993, S995, S996, S1007, S1032, S1050, S1085, S1180, S1195, S1240, S1247, S1250, S1256, S1257, S1275, S1337, S1340, S1351, S1364, S1381, S1393, S1399, S1407, S1425, S1429, S1435, S1447, S1503, S1504, S1631, S1642, S1660, S1669, S1698, S1768, S1771.
Threonine phosphorylated:
T82, T185, T243, T274, T279, T867, T871, T1349, T1508, T1564, T1567, T1798.
Tyrosine phosphorylated:
Y461, Y812, Y853.
Ubiquitinated:
K1183, K1197.
Distribution
Based on gene microarray analysis from the NCBI
Human Tissue Distribution
% Max Expression:
Mean Expression:
Number of Samples:
Standard Deviation:
% Max Expression:
Mean Expression:
Number of Samples:
Standard Deviation:
99
810
55
1014
20
166
28
274
24
196
23
156
44
356
174
523
100
814
48
648
5
41
148
38
26
214
58
383
95
775
72
872
74
603
34
469
11
90
150
89
13
105
57
165
85
694
291
613
6
49
69
139
40
327
20
737
12
98
45
208
13
110
30
172
11
88
150
111
24
197
41
339
51
412
184
624
56
458
220
635
22
176
41
289
6
45
47
34
9
72
27
54
80
654
43
906
14
117
41
167
100
815
105
2076
12
101
75
193
24
192
40
440
22
180
41
232
25
206
56
128
40
323
36
435
73
595
68
543
11
90
99
264
98
801
104
658
49
399
61
542
Evolution
Species Conservation
PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:
PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:
100
100
100
68.5
68.7
100
97
97.7
97
-
-
90
-
-
90
82.9
86.3
89.5
-
-
-
85.4
88.7
88
56.8
65.8
89
-
-
-
57.6
65.9
-
68.7
76.2
82
51
62
81
64.9
75
79
-
-
-
-
-
37
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
For a wider analysis go to PhosphoNET Evolution in PhosphoNET
Binding Proteins
Examples of known interacting proteins
hiddentext
| No. | Name – UniProt ID |
|---|---|
| 1 | RPS27A - P62988 |
| 2 | RPS27A - P62979 |
| 3 | CDHR2 - Q9BYE9 |
| 4 | SLC34A1 - Q06495 |
| 5 | DYNLL1 - P63167 |
| 6 | STK36 - Q9NRP7 |
| 7 | GCN1L1 - Q92616 |
| 8 | YWHAH - Q04917 |
Regulation
Activation:
NA
Inhibition:
NA
Synthesis:
NA
Degradation:
NA
Protein Kinase Specificity
Matrix of observed frequency (%) of amino acids in aligned protein substrate phosphosites
Matrix Type:
Predicted from the application of the Kinexus Kinase Substrate Predictor Version 2.0 algorithm, which was trained with over 10,000 kinase-protein substrate pairs and 8,000 kinase-peptide substrate pairs.
Domain #:
1
Disease Linkage
General Disease Association:
Cancer
Specific Cancer Types:
Breast cancer
Comments:
A translocation of the MAST2 may be involved in breast carcinoma and pre-invasive lesions.
Gene Expression in Cancers:
TranscriptoNET (www.transcriptonet.ca) analysis with mRNA expression data retrieved from the National Center for Biotechnology Information's Gene Expression Omnibus (GEO) database, which was normalized against 60 abundantly and commonly found proteins, indicated altered expression for this protein kinase as shown here as the percent change from normal tissue controls (%CFC) as supported with the Student T-test in the following types of human cancers: Barrett's esophagus epithelial metaplasia (%CFC= +74, p<0.021); Breast epithelial cell carcinomas (%CFC= +51, p<0.033); Lung adenocarcinomas (%CFC= +117, p<0.002); and Ovary adenocarcinomas (%CFC= +118, p<0.005).
Mutagenesis Experiments:
Insertional mutagenesis studies in mice have not yet revealed a role for this protein kinase in mouse cancer oncogenesis.
Mutation Rate in All Cancers:
Percent mutation rates per 100 amino acids length in human cancers: 0.05 % in 24494 diverse cancer specimens. This rate is only -30 % lower than the average rate of 0.075 % calculated for human protein kinases in general.
Mutation Rate in Specific Cancers:
Highest percent mutation rates per 100 amino acids length in human cancers: 0.34 % in 1052 large intestine cancers tested; 0.18 % in 805 skin cancers tested; 0.14 % in 569 stomach cancers tested; 0.11 % in 602 endometrium cancers tested; 0.1 % in 820 ovary cancers tested; 0.08 % in 1594 lung cancers tested; 0.05 % in 1270 liver cancers tested.
Frequency of Mutated Sites:
None > 2 in 20,253 cancer specimens
Comments:
Eight deletions (7 at F726fs*12 ) and 4 (2 at G727fs*14) insertions, and no complex mutations are noted on the COSMIC website.