Nomenclature
Short Name:
CKM
Full Name:
Creatine kinase M-type
Alias:
- CKMM
- Creatine kinase M chain
- Creatine kinase, M chain
- Creatine kinase, muscle
- KCRM
- M-CK
Classification
Type:
Protein-serine/threonine kinase
Group:
Atypical
Family:
CK
SubFamily:
NA
Specific Links
Structure
Mol. Mass (Da):
43101
# Amino Acids:
381
# mRNA Isoforms:
5
mRNA Isoforms:
93,497 Da (822 AA; P07332); 86,663 Da (764 AA; P07332-3); 85,466 Da (752 AA; P07332-4); 78,633 Da (694 AA; P07332-2); 43,101 Da (381 AA; P06732)
4D Structure:
1D Structure:
3D Image (rendered using PV Viewer):
PDB ID
Subfamily Alignment
Domain Distribution:
| Start | End | Domain |
|---|
Post-translation Modifications
For detailed information on phosphorylation of this kinase go to PhosphoNET
Acetylated:
K40, K41, K170, K247, K265, K365.
Serine phosphorylated:
S24, S128, S129, S136, S164, S178, S199, S224, S332, S372.
Threonine phosphorylated:
T35, T141, T166, T180, T313, T322, T327.
Tyrosine phosphorylated:
Y14, Y20, Y39, Y125, Y140, Y173, Y174, Y279.
Distribution
Based on gene microarray analysis from the NCBI
Human Tissue Distribution
% Max Expression:
Mean Expression:
Number of Samples:
Standard Deviation:
% Max Expression:
Mean Expression:
Number of Samples:
Standard Deviation:
55
2694
19
7131
0.3
15
8
5
0.4
22
1
0
4
207
40
402
5
226
14
221
0
2
37
1
3
171
19
329
81
3996
17
3423
5
230
10
213
0.7
33
42
54
0.7
36
12
33
9
459
105
537
0.1
6
12
3
0.2
12
7
5
0.3
15
9
8
0.1
3
7
1
2
76
98
344
4
180
8
236
100
4928
64
5155
6
281
56
368
0.3
16
8
6
0.2
9
12
8
3
157
10
65
8
392
6
561
0.2
12
8
5
14
707
24
972
8
383
15
677
0.9
44
8
37
0.3
16
7
5
1.3
62
14
38
11
564
18
412
25
1250
21
2483
3
132
39
402
9
466
31
474
1
47
22
40
Evolution
Species Conservation
PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:
PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:
100
100
100
96.3
97.3
100
80.5
90.2
-
-
-
97
-
-
-
75.1
77.4
96
-
-
-
96.5
98.9
97
79.5
89.5
96
-
-
-
70.3
78.2
-
90.2
95.8
90
88.7
95.5
90
88.7
94.2
85
-
-
-
42.5
58.7
-
40.4
59
-
37.5
55.3
-
22.4
27.8
-
-
-
-
79.5
89.5
-
-
-
-
-
-
-
-
-
-
-
-
-
For a wider analysis go to PhosphoNET Evolution in PhosphoNET
Regulation
Activation:
NA
Inhibition:
NA
Synthesis:
NA
Degradation:
NA
Disease Linkage
General Disease Association:
Cardiovascular, blood, and neuronal disorders
Specific Diseases (Non-cancerous):
Myocardial infarction; Hemophilia B; Neuroacanthocytosis
Comments:
In 59 families with myotonic dystrophy (DM), very close linkage of the disease and the polymorphism at the CKMM locus has been found.
Gene Expression in Cancers:
TranscriptoNET (www.transcriptonet.ca) analysis with mRNA expression data retrieved from the National Center for Biotechnology Information's Gene Expression Omnibus (GEO) database, which was normalized against 60 abundantly and commonly found proteins, indicated altered expression for this protein kinase as shown here as the percent change from normal tissue controls (%CFC) as supported with the Student T-test in human Breast epithelial hyperplastic enlarged lobular units (HELU) (%CFC= -46, p<0.041). The COSMIC website notes an up-regulated expression score for CKM in diverse human cancers of 247, which is 47% lower than the average score of 462 for the human protein kinases. The down-regulated expression score of 0 for this protein kinase in human cancers was 100% lower than the average score of 60 for the human protein kinases.
Mutagenesis Experiments:
Insertional mutagenesis studies in mice have not yet revealed a role for this protein kinase in mouse cancer oncogenesis.
Mutation Rate in All Cancers:
Percent mutation rates per 100 amino acids length in human cancers: 0.08 % in 24523 diverse cancer specimens. This rate is very similar (+ 7% higher) to the average rate of 0.075 % calculated for human protein kinases in general.
Mutation Rate in Specific Cancers:
Highest percent mutation rates per 100 amino acids length in human cancers: 0.49 % in 1240 large intestine cancers tested; 0.43 % in 864 skin cancers tested; 0.22 % in 589 stomach cancers tested; 0.15 % in 1608 lung cancers tested.
Frequency of Mutated Sites:
None > 3 in 19,779 cancer specimens
Comments:
Only 1 deletion and 1 insertion and no complex mutations are noted on the COSMIC website.