Nomenclature
Short Name:
GTF2F1
Full Name:
General transcription factor IIF subunit 1
Alias:
- BTF4
- TF2F1
- TFIIF
- Transcription initiation factor IIF alpha subunit
- Transcription initiation factor IIF subunit alpha; Transcription nitiation factor RAP74
- General transcription factor IIF polypeptide 1 74 kDa subunit protein
- General transcription factor IIF, polypeptide 1 (74kD subunit)
- General transcription factor IIF, polypeptide 1, 74kDa
- RAP74
- T2FA
Classification
Type:
Protein-serine/threonine kinase
Group:
Atypical
Family:
SubFamily:
NA
Specific Links
| Kinexus Products: | GTF2F1 |
Structure
Mol. Mass (Da):
58240
# Amino Acids:
517
# mRNA Isoforms:
1
mRNA Isoforms:
58,240 Da (517 AA; P35269)
4D Structure:
1D Structure:
3D Image (rendered using PV Viewer):
PDB ID
Subfamily Alignment
Domain Distribution:
| Start | End | Domain |
|---|
Kinexus Products
Click on entries below for direct links to relevant products from Kinexus for this protein kinase.
hiddentext
Post-translation Modifications
For detailed information on phosphorylation of this kinase go to PhosphoNET
Acetylated:
K315, K328, K407, K412, K421, K466, K471.
Serine phosphorylated:
S217, S218, S221, S224, S253, S261, S305, S307, S308, S311, S341, S342, S345, S347, S348, S351, S355, S358, S359, S377, S380, S381, S385, S391, S396, S398, S399, S415, S431, S433, S436, S442, S449.
Threonine phosphorylated:
T154, T156, T331, T368, T389, T397, T427, T437, T445, T446, T455.
Tyrosine phosphorylated:
Y57.
Ubiquitinated:
K55, K169, K183, K510.
Distribution
Based on gene microarray analysis from the NCBI
Human Tissue Distribution
% Max Expression:
Mean Expression:
Number of Samples:
Standard Deviation:
% Max Expression:
Mean Expression:
Number of Samples:
Standard Deviation:
62
954
42
1042
9
139
20
79
15
234
12
131
29
438
127
520
63
962
35
732
5
76
118
122
12
190
43
351
100
1531
52
3603
42
637
24
447
11
162
112
148
9
138
39
155
52
789
211
632
9
134
45
116
10
157
15
115
23
351
30
549
9
131
22
71
12
191
131
84
12
185
21
140
6
86
120
62
39
600
162
620
11
167
27
131
10
160
33
108
14
210
23
153
17
261
23
133
12
186
27
122
50
764
75
707
9
141
48
94
13
206
23
160
14
220
23
141
15
230
42
88
47
716
30
770
48
742
51
789
4
60
51
43
52
790
83
663
22
339
48
307
Evolution
Species Conservation
PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:
PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:
100
100
100
99.4
99.8
99
98.4
99.2
98.5
-
-
95
-
-
-
78.2
80.8
93
-
-
-
91.3
93.6
93
91.6
93.8
93
-
-
-
-
-
-
-
-
-
75.7
88.7
74
60.1
70.7
72
-
-
-
37
57.1
-
39.8
58
-
32.1
51.2
-
37.7
57.6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
For a wider analysis go to PhosphoNET Evolution in PhosphoNET
Regulation
Activation:
NA
Inhibition:
NA
Synthesis:
NA
Degradation:
NA
Disease Linkage
Gene Expression in Cancers:
The COSMIC website notes an up-regulated expression score for GTF2F1 in diverse human cancers of 446, which is close to the average score of 462 for the human protein kinases. The down-regulated expression score of 236 for this protein kinase in human cancers was 3.9-fold of the average score of 60 for the human protein kinases.
Mutagenesis Experiments:
Insertional mutagenesis studies in mice have not yet revealed a role for this protein kinase in mouse cancer oncogenesis. It can be inhibited by mutation of S385 and T389.
Mutation Rate in All Cancers:
Percent mutation rates per 100 amino acids length in human cancers: 0.06 % in 24549 diverse cancer specimens. This rate is only -21 % lower than the average rate of 0.075 % calculated for human protein kinases in general.
Mutation Rate in Specific Cancers:
Highest percent mutation rates per 100 amino acids length in human cancers: 0.42 % in 1240 large intestine cancers tested; 0.29 % in 603 endometrium cancers tested; 0.18 % in 864 skin cancers tested; 0.14 % in 555 stomach cancers tested; 0.11 % in 548 urinary tract cancers tested; 0.08 % in 1609 lung cancers tested.
Frequency of Mutated Sites:
None > 3 in 19,805 cancer specimens
Comments:
Only 4 deletions, 2 insertions, and no complex mutations are noted on the COSMIC website.