Nomenclature
Short Name:
FASTK
Full Name:
Fas-activated serine-threonine kinase
Alias:
- EC 2.7.11.8
- FAST
- FAST kinase
- Kinase FASTK
Classification
Type:
Protein-serine/threonine kinase
Group:
Atypical
Family:
FAST
SubFamily:
NA
Structure
Mol. Mass (Da):
61,104
# Amino Acids:
549
# mRNA Isoforms:
3
mRNA Isoforms:
61,104 Da (549 AA; Q14296); 58,181 Da (522 AA; Q14296-3); 45,852 Da (408 AA; Q14296-2)
4D Structure:
Interacts with TIA-1.
1D Structure:
Subfamily Alignment
Domain Distribution:
| Start | End | Domain |
|---|---|---|
| 277 | 444 | FAST_Leu-rich |
| 477 | 535 | RAP |
| 353 | 444 | FAST2 |
Post-translation Modifications
For detailed information on phosphorylation of this kinase go to PhosphoNET
Serine phosphorylated:
S41, S45, S94, S127, S497.
Tyrosine phosphorylated:
Y532.
Distribution
Based on gene microarray analysis from the NCBI
Human Tissue Distribution
% Max Expression:
Mean Expression:
Number of Samples:
Standard Deviation:
% Max Expression:
Mean Expression:
Number of Samples:
Standard Deviation:
49
1010
42
1029
7
153
23
168
5
100
22
132
24
498
192
881
48
984
35
717
6
117
116
170
12
236
46
491
45
916
65
1215
32
650
27
524
9
179
125
189
29
604
91
1732
44
904
245
735
7
140
55
147
4
80
18
112
8
166
43
225
9
192
22
135
10
215
221
161
7
143
36
199
31
637
192
1645
32
651
165
633
7
145
40
175
10
205
46
274
9
186
33
154
22
449
88
1376
9
193
40
288
57
1179
88
2610
6
131
61
124
7
149
36
193
6
113
36
121
11
224
42
183
32
659
30
667
39
804
51
747
100
2052
90
2766
41
838
83
710
21
430
48
340
Evolution
Species Conservation
PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:
PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:
100
100
100
99.3
99.5
100
93.1
93.6
98
-
-
92
-
-
93
90
92.2
94
-
-
-
83.8
88.3
89.5
22.6
35.7
91
-
-
-
-
-
-
21.6
35.4
54
-
-
-
-
-
47
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
For a wider analysis go to PhosphoNET Evolution in PhosphoNET
Binding Proteins
Examples of known interacting proteins
hiddentext
| No. | Name – UniProt ID |
|---|---|
| 1 | TIA1 - P31483 |
| 2 | CRK - P46108 |
| 3 | RBPMS - Q93062 |
Regulation
Activation:
NA
Inhibition:
NA
Synthesis:
NA
Degradation:
NA
Disease Linkage
General Disease Association:
Developmental disorders
Specific Diseases (Non-cancerous):
Autism spectrum disorder (ASD)
Comments:
Autism Spectrum Disorder (ASD) is a rare condition characterized by social and communication difficulties, repetitive behaviour, and stereotype patterns of behaviour. ASD can affect brain, eye, and amygdala tissues.
Gene Expression in Cancers:
TranscriptoNET (www.transcriptonet.ca) analysis with mRNA expression data retrieved from the National Center for Biotechnology Information's Gene Expression Omnibus (GEO) database, which was normalized against 60 abundantly and commonly found proteins, indicated altered expression for this protein kinase as shown here as the percent change from normal tissue controls (%CFC) as supported with the Student T-test in the following types of human cancers: Breast epithelial cell carcinomas (%CFC= +49, p<0.055); Large B-cell lymphomas (%CFC= +60, p<0.0001); Ovary adenocarcinomas (%CFC= +116, p<0.028); Prostate cancer - metastatic (%CFC= +56, p<0.0001); Prostate cancer - primary (%CFC= -56, p<0.0001); Skin melanomas - malignant (%CFC= +107, p<0.0002); and Uterine leiomyomas from fibroids (%CFC= +62, p<0.051). The COSMIC website notes an up-regulated expression score for FASTK in diverse human cancers of 604, which is 1.3-fold of the average score of 462 for the human protein kinases. The down-regulated expression score of 35 for this protein kinase in human cancers was 0.6-fold of the average score of 60 for the human protein kinases.
Mutagenesis Experiments:
Insertional mutagenesis studies in mice have not yet revealed a role for this protein kinase in mouse cancer oncogenesis.
Mutation Rate in All Cancers:
Percent mutation rates per 100 amino acids length in human cancers: 0.04 % in 24751 diverse cancer specimens. This rate is -41 % lower than the average rate of 0.075 % calculated for human protein kinases in general.
Mutation Rate in Specific Cancers:
Highest percent mutation rates per 100 amino acids length in human cancers: 0.22 % in 589 stomach cancers tested; 0.17 % in 864 skin cancers tested; 0.16 % in 1270 large intestine cancers tested; 0.09 % in 833 ovary cancers tested; 0.06 % in 603 endometrium cancers tested.
Frequency of Mutated Sites:
None >3 in 20,034 cancer specimens
Comments:
Only 3 deletions, 1 insertion, and no complex mutations are noted on the COSMIC website.