KinaseNET

Nomenclature

Short Name:

PKACa

Full Name:

cAMP-dependent protein kinase, alpha-catalytic subunit

Alias:

Alpha-catalytic subunit
Kinase PKA C-alpha
PKA C-alpha
PKA-alpha
PKACA; Protein kinase, cAMP-dependent, catalytic, alpha
cAMP-dependent protein kinase
cAMP-dependent protein kinase, alpha-catalytic subunit
cAPKa
EC 2.7.11.11
KAPCA

Classification

Type:

Protein-serine/threonine kinase

Group:

AGC

Family:

PKA

SubFamily:

Specific Links

Entrez-Gene Entry:	5566
Entrez-Protein Entry:	NP_002721
GeneCards Entry:	PKACA
KinBASE Entry:	PKACA
OMIM Entry:	601639
Pfam Entry:	P17612
PhosphoNET Entry:	P17612
Phosphosite Plus Entry:	637
Protein Data Bank Entry:	2GU8
ScanSite Entry:	P17612
Source Entry:	PRKACA
UCSD-Nature Entry:	A001914
UniProt Entry:	P17612
Kinexus Products:	PKACa cAMP-dependent protein kinase catalytic subunit alpha T196+T198 phosphosite-specific antibody AB-PK782 CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide - Powder PE-01ACP95 KEMPtide KinSub - PKA protein kinase substrate peptide - Powder PE-01ADW95 PKASubtide - PKACb (PRKACB) protein kinase substrate peptide - Powder PE-01BIF95 CREB1 (123-135) KinSub, biotinyl. - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide - Powder PE-03AAF90 cAMP-dependent protein kinase catalytic subunit alpha (K193-G201, human) pT196+pT198 phosphopeptide - Powder PE-04AOQ85

General Links

Structure

Mol. Mass (Da):

40590

# Amino Acids:

351

# mRNA Isoforms:

mRNA Isoforms:

40,590 Da (351 AA; P17612); 39,822 Da (343 AA; P17612-2)

4D Structure:

A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.;; C2R2 - a dimer of two catalytic subunits and 2 regulatory subunits. RI (uniprot), RII (uniprot)

3D Structure:

Download QuickTime Animation

1D Structure:

Retrieve Gene Sequence
Retrieve Full Protein Sequence
Retrieve Catalytic Domain Sequence

3D Image (rendered using PV Viewer):

PDB ID

4WB5

Subfamily Alignment

Domain Distribution:

Start	End	Domain
44	298	Pkinase
299	351	Pkinase_C

Kinexus Products

Click on entries below for direct links to relevant products from Kinexus for this protein kinase.

hiddentext

○	cAMP-dependent protein kinase catalytic subunit alpha T196+T198 phosphosite-specific antibody AB-PK782
○	CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide - Powder PE-01ACP95
○	KEMPtide KinSub - PKA protein kinase substrate peptide - Powder PE-01ADW95
○	PKASubtide - PKACb (PRKACB) protein kinase substrate peptide - Powder PE-01BIF95
○	CREB1 (123-135) KinSub, biotinyl. - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide - Powder PE-03AAF90
○	cAMP-dependent protein kinase catalytic subunit alpha (K193-G201, human) pT196+pT198 phosphopeptide - Powder PE-04AOQ85

Post-translation Modifications

For detailed information on phosphorylation of this kinase go to PhosphoNET

Acetylated:

K267, K280.

Myristoylated:

G2.

Serine phosphorylated:

S11, S15, S54-, S140, S260, S264, S339+.

Threonine phosphorylated:

T49-, T52, T89, T196+, T198+, T202-.

Tyrosine phosphorylated:

Y205, Y331.

Ubiquitinated:

K17, K24, K84, K93, K190, K267, K280, K286, K320.

Distribution

Based on gene microarray analysis from the NCBI

Human Tissue Distribution

% Max Expression:

Mean Expression:

Number of Samples:

Standard Deviation:

81

1256

29

1789
18

279

18

287
21

328

11

292
27

412

109

522
45

702

25

607
84

1299

89

3863
30

470

39

639
57

888

49

870
32

494

17

406
14

217

107

232
15

226

36

240
33

512

216

644
9

140

33

173
7

111

13

104
7

106

21

102
22

337

16

405
7

110

290

860
15

240

20

285
18

282

114

389
30

460

109

536
16

254

29

336
16

255

32

306
16

244

29

211
24

369

22

429
10

151

30

182
100

1551

61

2821
9

137

36

177
12

183

21

166
14

222

21

242
7

101

28

73
22

341

24

288
61

952

36

1277
4

62

73

149
45

696

62

672
24

375

35

773

Evolution

Species Conservation

PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:

100

100

100
81.5

82

0
51.3

56.7

-
-

-

99
-

-

98
93.2

95.4

99
-

-

-
98

99.4

98
98

99.2

98
-

-

-
-

-

-
81.4

84.7

95
35.3

54.1

93
34.9

53.1

89.5
-

-

-
82.4

90.1

82
-

-

-
68.6

78.5

83
-

-

-
-

-

-
-

-

-
-

-

-
-

-

-
45.5

67.1

53
-

-

-

For a wider analysis go to PhosphoNET Evolution in PhosphoNET

Regulation

Activation:

Activated by binding of two cAMP molecules to each of the two associated regulatory subunits in the PKA holoenzyme. Binding of cAMP induces dissociation of the two active catalytic subunits. Phosphorylation of Thr-198 increases phosphotransferase activity. Phosphorylation of S338 plays a key role in stabilizing PKA.

Inhibition:

Synthesis:

Degradation:

Known Upstream Kinases

For further details on these substrates click on the Substrate Short Name or UniProt ID. Phosphosite Location is hyperlinked to PhosphoNET predictions.

Based on in vitro and/or in vivo phosphorylation data

Kinase Short Name	UniProt ID (Human)	Phosphosite Location	Phosphosite Sequence	Effect of Phosphorylation

PKACa	P17612	S11	AAAAKKGSEQESVKE
PKACa	P17612	S140	LRRIGRFSEPHARFY
PDK1	O15530	T198	RVKGRTWTLCGTPEY	+
PKACa	P17612	T198	RVKGRTWTLCGTPEY	+
PDK1	O15530	S339	EEEEIRVSINEKCGK	+
PKACa	P17612	S339	EEEEIRVSINEKCGK	+

Known Downstream Substrates

For further details on these substrates click on the Substrate Short Name or UniProt ID. Phosphosite Location is hyperlinked to PhosphoNET predictions.

Based on in vitro and/or in vivo phosphorylation data

Substrate Short Name	UniProt ID (Human)	Phosphosite Location	Phosphosite Sequence	Effect of Phosphorylation

Desmin	P17661	S44	AGFGSKGSSSSVTSR
Desmin	P17661	S59	VYQVSRTSGGAGGLG
Desmin	P17661	S81	GTTRTPSSYGAGELL
Desmin	P17661	T16	RVSSYRRTFGGAPGF
Desmin	P17661	T75	LRASRLGTTRTPSSY
Desmoplakin	P15924	S2849	RSGSRRGSFDATGNS
DJ-1	Q99497	S155	KDGLILTSRGPGTSF
DJ-1	Q99497	T154	EKDGLILTSRGPGTS
Doublecortin	O43602	S128	TRTLQALSNEKKAKK
DRP1	O00429	S637	VPVARKLSAREQRDC
DUOX1	Q9NRD9	S1217	SHHFRRRSFRGFWLT
DUOX1	Q9NRD9	S955	KDLCRRASYISQDMI
Dynactin-1	Q14203	S19	TPSGSRMSAEASARP
eEF1B	P24534	S105	DDIDLFGSDDEEESE
eEF1B	P24534	S140	PALVAKSSILLDVKP
eEF1G	P26641	S298	DEFKRKYSNEDTLSV
eEF2K	O00418	S366	SPRVRTLSGSRPPLL	-
eEF2K	O00418	S500	RLHLPRASAVALEVQ
eIF2B	P20042	S218	RVGTKKTSFVNFTDI
Emerin	P50402	S49	ETQRRRLSPPSSSAA
En-2	P19622	S276	LTEQRRQSLAQELSL
eNOS	P29474	S1177	TSRIRTQSFSLQERQ	+
eNOS	P29474	S615	SYKIRFNSISCSDPL
eNOS	P29474	S633	WRRKRKESSNTDSAG
eNOS	P29474	T495	TGITRKKTFKEVANA
ERa (ESR1)	P03372	S236	IDKNRRKSCQACRLR	?
ERa (ESR1)	P03372	S305	IKRSKKNSLALSLTA	+
ErbB2 (HER2)	P04626	T686	QQKIRKYTMRRLLQE	-
ERM	P41161	S367	PPYQRRGSLQLWQFL
ETV1 (ER81)	P50549	S191	HRFRRQLSEPCNSFP	+
ETV1 (ER81)	P50549	S216	PMYQRQMSEPNIPFP	+
ETV1 (ER81)	P50549	S334	PTYQRRGSLQLWQFL
Ezrin	P15311	S66	LKLDKKVSAQEVRKE
FAK (PTK2) iso5	Q05397	S840	LKPDVRLSRGSIDRE
FETUB	Q9UGM5	S303	SKAGPRGSVQYLPDL
FKBP9	O95302	S277	NCERISQSGDFLRYH
FLNA	P21333	S2152	TRRRRAPSVANVGSH
FLNA	P21333	S2523	VTGPRLVSNHSLHET
FLNA	P21333	S2615	HVGSRLYSVSYLLKD
FLNA	P21333	S343	NDKNRTFSVWYVPEV
FLNA	P21333	T2336	SGDARRLTVSSLQES
FLNC	Q14315	T2412	SDDARRLTVTSLQET
FRAT1	Q92837	S188	RLQQRRGSQPETRTG
Fyn	P06241	S21	LTEERDGSLNQSSGY	+
Ga(13)	Q14344	T203	ILLARRPTKGIHEYD
GABBR2	O75899	S893	EHIQRRLSLQLPILH
GAD67	Q99259	T91	RDARFRRTETDFSNL
GANAB	Q14697	S916	GSPESRLSFQHDPET
GATA3	P23771	S308	IKPKRRLSAARRAGT
GATA4	P43694	S262	IKPQRRLSASRRVGL
GBF1	Q92538	S174	ICFEMRLSELLRKSA
GCAP2	Q9UMX6	S197	LAQQRRKSAMF____
GFAP	P14136	S13	ITSAARRSYVSSGEM
GFAP	P14136	S38	LGPGTRLSLARMPPP
GFAP	P14136	S8	MERRRITSAARRSYV
GFAP	P14136	T7	_MERRRITSAARRSY
GFAT	Q06210	S204	AVGTRRGSPLLIGVR
GFAT	Q06210	S234	TARTQIGSKFTRWGS
GHF1	P28069	S115	KQELRRKSKLVEEPI
GHF1	P28069	T220	RKRKRRTTISIAAKD
GIRK1	P48549	S385	NSKERHNSVECLDGL
GLI1	P08151	S544	VSLERRSSSSSSISS
GLI1	P08151	S560	YTVSRRSSLASPFPP
GLI1	P08151	S640	AGVTRRASDPAQAAD
GLI1	P08151	T374	PGCTKRYTDPSSLRK
GLUD1	P00367	T206	EKITRRFTMELAKKG
GluR1	P42261	S849	FCLIPQQSINEAIRT
GluR1	P42261	S863	TSTLPRNSGAGASSG
GluR4	P48058	S862	IRNKARLSITGSVGE
GluR6	Q13002	S856	NAQLEKRSFCSAMVE
GluR6	Q13002	S868	MVEELRMSLKCQRRL
GLUT2	P11168	S491	VPETKGKSFEEIAAE
GLUT2	P11168	S503	AAEFQKKSGSAHRPK
GLUT2	P11168	S505	EFQKKSGSAHRPKAA
GLUT4	P14672	S488	AAFHRTPSLLEQEVK
GMFb	P60983	S82	QHDDGRVSYPLCFIF
GMFb	P60983	T26	KFRFRKETNNAAIIM
GNMT	Q14749	S9	DSVYRTRSLGVAAEG
GPLD1	P80108	T309	NDFHRNLTTSLTESV	-
GRK7	Q8WTQ7	S23	YLQARKPSDCDSKEL	-
GRK7	Q8WTQ7	S36	ELQRRRRSLALPGLQ	-
GSK3a	P49840	S21	SGRARTSSFAEPGGG	-
GSK3b	P49841	S9	SGRPRTTSFAESCKP	-
GSTA4	O15217	S189	QEYTVKLSNIPTIKR
GSTP1	P09211	S184	SAYVGRLSARPKLKA
GYS1	P13807	S8	MPLNRTLSMSSLPGL	-
GYS2	P54840	S8	MLRGRSLSVTSLGGL	-
H-Ras-1	P01112	S177	KLNPPDESGPGCMSC
H11 (HSPB8)	Q9UJY1	S57	DWALPRLSSAWPGTL	?
H1D	P16403	S36	GGTPRKASGPPVSEL
H1F1	Q02539	T132	VATKTKATGASKKLK
H1R	P35367	S398	WKRLRSHSRQYVSGL
H2B	P33778	S33	DGKKRKRSRKESYSI
H2B	P33778	S37	RKRSRKESYSIYVYK
H3.1	P68431	S11	TKQTARKSTGGKAPR	+
H3.2	P84228	S11	TKQTARKSTGGKAPR
H3.2	P84228	S29	ATKAARKSAPATGGV
HDAC8	Q9BY41	S39	AKIPKRASMVHSLIE
HePTP	P35236	S44	RLQERRGSNVALMLD	-
HMG-CoA reductase	P04035	S872	SHMIHNRSKINLQDL
HMGN1 (HMG14)	P05114	S7	_PKRKVSSAEGAAKE
hnRNP A1	P09651	S199	SQRGRSGSGNFGGGR	-
hnRNP D	Q14103	S87	SNSSPRHSEAATAQR
hnRNP I	P26599	S16	AVGTKRGSDELFSTC
HPK1 (MAP4K1)	Q92918	S171	ATLARRLSFIGTPYW	+
HSL	Q05469	S565	ASATVRVSRLLSLPP
HSL	Q05469	S853	IAEPMRRSVSEAALA
HSL	Q05469	S950	EGFHPRRSSQGATQM
HSL	Q05469	S951	GFHPRRSSQGATQMP
HSP20	O14558	S16	PSWLRRASAPLPGLS	?
HSP27	P04792	S15	FSLLRGPSWDPFRDW	?
HSP27	P04792	S78	PAYSRALSRQLSSGV	+
HSP27	P04792	S82	RALSRQLSSGVSEIR	?
HSP27	P04792	T143	RCFTRKYTLPPGVDP
HSP90AA1	P07900	S211	KEIVKKHSQFIGYPI
HSP90AA1	P07900	S263	PEIEDVGSDEEEEKK
HSP90B	P08238	S452	STNRRRLSELLRYHT
ICLN	P54105	S45	YIAESRLSWLDGSGL
IP3R1 (IP3 receptor)	Q14643	S1598	RNAARRDSVLAASRD
IP3R1 (IP3 receptor)	Q14643	S1764	RPSGRRESLTSFGNG
IP3R3	Q14573	S1832	TTKGRVASFSIPGSS
IP3R3	Q14573	S916	GGKNVRRSIQGVGHM
IP3R3	Q14573	S934	MVLSRKQSVFSAPSL
ITCH	Q96J02	S450	GWEKRTDSNGRVYFV
ITCH	Q96J02	T464	VNHNTRITQWEDPRS
ITGA4	P13612	S1027	QEENRRDSWSYINSK
ITGB4	P16144	S1364	PSGSQRPSVSDDTGC
ITIH4	Q14624	S225	TLSQQQKSPEQQETV
KCNJ13	O60928	S287	EICQRRTSYLPSEIM
KCNJ8	Q15842	S385	NSLRKRNSMRRNNSM
KCNJ8	Q15842	T234	IQVVKKTTTPEGEVV
KCNK18	Q7Z418	S252	QAMERSNSCPELVLG
KCNN2	Q9H2S1	S135	FEKRKRLSDYALIFG
KCNN2	Q9H2S1	S464	QAIHQLRSVKMEQRK
KCNN2	Q9H2S1	S567	SSSRRRRSSSTAPPT
KCNN2	Q9H2S1	S568	SSRRRRSSSTAPPTS
KCNN2	Q9H2S1	S569	SRRRRSSSTAPPTSS
KCNN4	O15554	S314	YTKEMKESAARVLQE
KCNN4	O15554	S334	KHTRRKESHAARRHQ
KCNN4	O15554	T329	AWMFYKHTRRKESHA
KDELR1	P24390	S209	VLKGKKLSLPA____
Kir5.1	Q9NPI9	S416	LNRISMESQM_____
Kir6.2	Q14654	T224	MQVVRKTTSPEGEVV
Kv1.2	P16389	S449	PDLKKSRSASTISKS
Kv1.4	P22459	S227	FFDRNRPSFDAILYY
Kv1.4	P22459	T601	LKKFRSSTSSSLGDK
Kv4.2	Q9NZV8	T38	PRQERKRTQDALIVL
L-plastin	P13796	S5	___MARGSVSDEEMM
Lamin A,C	P02545	S5	___METPSQRRATRS
Lasp-1	Q14847	S146	MEPERRDSQDGSSYR
Lasp-1	Q14847	S99	KNKGKGFSVVADTPE
Lck	P06239	S42	TLLIRNGSEVRDPLV
LKB1 (STK11)	Q15831	S428	SSKIRRLSACKQQ__	+
LPH (Lactase-phlorizin hydrolase)	P09848	S1917	KQGKTQRSQQELSPV
LPP	Q93052	S116	TLEERRSSLDAEIDS
LRP1	Q07954	S4520	GGHGSRHSLASTDEK
LRPAP1	P30533	S242	LDRLRRVSHQGYSTE
MAP2 iso3	P11137	T1577	SSSARRTTRSEPIRR
MAPKAPK5	Q8IW41	S115	GELFHRISQHRHFTE	?
MARCKS	P29966	S159	KKKKKRFSFKKSFKL
MARK3	P27448	T507	GGMTRRNTYVCSERT
MAZ	P56270	S186	ALEKKTKSKGPYICA
MAZ	P56270	T385	KDRLRAHTVRHEEKV
MC4R	P32245	T312	RSQELRKTFKEIICC
MCAM	P43121	S85	VHQGKGQSEPGEYEH
MCAM	P43121	S93	GEYEQRLSLQDRGAT
MEF2C	Q06413	S121	EDKYRRASEELDGLF
MEF2C	Q06413	S190	QPALQRNSVSPGLPQ
MEF2C	Q06413	T20	DERNRQVTFTKRKFG	+
Merlin	P35240	S10	GAIASRMSFSSLKRK
Merlin	P35240	S518	DTDMKRLSMEIEKEK
mGluR2	Q14416	S675	AQRPRFISPASQVAI
mGluR2	Q14416	S843	GSAAARASSSLGQGS
mGluR3	Q14832	S843	RLHLNRFSVSGTGTT
mGluR7	Q14831	S862	NVQKRKRSFKAVVTA
Moesin	P26038	T558	LGRDKYKTLRQIRQG
Mos	P00540	S25	SVDARPCSSPSELPA	-
MRLC1 (MYL9)	P24844	S20	KRPQSATSNVFAMFD
MRLC1 (MYL9)	P24844	T19	KKRPQSATSNVFAMF
MST3 (STK24)	Q9Y6E0	T18	ALNKRRATLPHPGGS	+
Mucolipin 1	Q9GZU1	S557	SGKFRRGSGSACSLL
Mucolipin 1	Q9GZU1	S559	KFRRGSGSACSLLCC
Myb	P10242	S116	KYGPKRWSVIAKHLK
MYBPC3	Q14896	T274	LLSAFRRTSLAGGGR
Myf-6	P23409	S220	SSSLRCLSSIVDSIS
Myf-6	P23409	S221	SSLRCLSSIVDSISS
Myf-6	P23409	S89	CKTCKRKSAPTDRRK
MYH11	P35749	T1763	SDRVRKATQQAEQLS
MYLK1 (smMLCK)	Q15746	S1388	LATCRSTSFNVQDLL
MYLK1 (smMLCK)	Q15746	S1760	RAIGRLSSMAMISGL
MYLK1 (smMLCK)	Q15746	S1773	GLSGRKSSTGSPTSP
MYLK1 (smMLCK)	Q15746	S1776	GRKSSTGSPTSPINA
MYLK1 (smMLCK)	Q15746	S1779	SSTGSPTSPLNAEKL
MYLK1 (smMLCK)	Q15746	S815	SLMLQNSSARALPRG	-
Myomesin 1	P52179	S482	ARLKSPLSTLDWTVI
Myomesin 2	P54296	S76	RVCAKRVSTQEDEEQ
MYPT1	O14974	S695	QARQSRRSTQGVTLT
MYPT1	O14974	S852	RPREKRRSTGVSFWT
nAChRA4	P43681	S362	LLLMKRPSVVKDNCR
nAChRA4	P43681	S467	LAKARSLSVQHMSSP
nAChRA4	P43681	S486	EGGVRCRSRSIQYCV
nAChRA7	P36544	S365	QHKQRRCSLASVEMS
NCOA3 (SRC-3)	Q9Y6Q9	S857	PPYNRAVSLDSPVSV	+
NDRG1	Q92597	S330	LMRSRTASGSSVTSL
NDRG1	Q92597	T366	GTRSRSHTSEGAHLD
NDUFS3	O75489	S59	DVAHKQLSAFGEYVA
NEDD4L	Q96PU5	S342	SSRLRSCSVTDAVAE	-
NEDD4L	Q96PU5	S448	IRRPRSLSSPTVTLS	?
NEDD4L	Q96PU5	T367	AGRARSSTVTGGEEP
Neurabin 1	Q9ULJ8	S460	ANRKIKFSSAPIKVF
NF-E2	Q16621	S170	TEAGRRRSEYVEMYP
NFAT2	O95644	S245	PSTSPRASVTEESWL
NFAT2	O95644	S269	PCNKRKYSLNGRQPP
NFAT2	O95644	S294	PHGSPRVSVTDDSWL
NFE2L1	Q14494	S599	PSALKKGSKEKQADF
NFkB-p105	P19838	S337	FVQLRRKSDLETSEP
NFkB-p65	Q04206	S276	SMQLRRPSDRELSEP
NFL (Neurofilament L)	P07196	S13	YEPYYSTSYKRRYVE
NFL (Neurofilament L)	P07196	S3	MSSFSYEPYY_____
NFL (Neurofilament L)	P07196	S42	TARSAYSSYSAPVSS
NFL (Neurofilament L)	P07196	S435	QTSSYLMSTRSFPSY
NFL (Neurofilament L)	P07196	S50	YSAPVSSSLSVRRSY
NFM (Neurofilament M)	P07197	S1	_______SYTLDSLG
NFM (Neurofilament M)	P07197	S22	RVTETRSSFSRVSGS
NFM (Neurofilament M)	P07197	S45	SWSRGSPSTVSSSYK
NFM (Neurofilament M)	P07197	S614	EKPEKAKSPVPKSPV
NFM (Neurofilament M)	P07197	S619	AKSPVPKSPVEEKGK
NFM (Neurofilament M)	P07197	S735	PEKKKAESPVKEEAV
NHE3	P48764	S555	AEGERRGSLAFIRSP
NHE3	P48764	S607	SLEQRRRSIRDAEDM
NHERF	O14745	S76	ETHQQVVSRIRAALN
NIPP-1	Q12972	S178	TAHNKRISTLTIEEG
NIPP-1	Q12972	S199	PKRKRKNSRVTFSED
NMDAR1	Q05586	S897	SFKRRRSSKDTSTGG
NMDAR2C	Q14957	S1227	PCTWRRISSLESEV
NOLC1	Q14978	S622	KKGEKRASSPFRRVR
NOLC1	Q14978	S623	KGEKRASSPFRRVRE
NR5A2	O00482	S510	LPEIRAISMQAEEYL	+
Nur77	P22736	S341	KEVVRTDSLKGRRGR
Nur77	P22736	S351	GRRGRLPSKPKQPPD	-
p47phox	P14598	S320	QRSRKRLSQDAYRRN	?
p47phox	P14598	S328	QDAYRRNSVRFLQQR	+
p47phox	P14598	S359	EERQTQRSKPQPAVP	+
p47phox	P14598	S370	PAVPPRPSADLILNR	+
p53	P04637	S378	SKKGQSTSRHKKLMF	+
PAH	P00439	S16	PGLGRKLSDFGQETS
PAK1	Q13153	T423	PEQSKRSTMVGTPYW	+
PAWR	Q96IZ0	T163	LREKRRSTGVVNIPA
PCTAIRE1 (CDK16, PCTK1)	Q00536	S110	NHPPRKISTEDINKR
PCTAIRE1 (CDK16, PCTK1)	Q00536	S119	EDINKRLSLPADIRL	-
PCTAIRE1 (CDK16, PCTK1)	Q00536	S12	KKIKRQLSMTLRGGR
PCTAIRE1 (CDK16, PCTK1)	Q00536	S153	SRRLRRVSLSEIGFG	-
PCTAIRE3 (CDK18, PCTK3)	Q07002	S12	KNFKRRFSLSVPRTE	+
PCTAIRE3 (CDK18, PCTK3)	Q07002	S130	SRMSRRASLSDIGFG	+
PCTAIRE3 (CDK18, PCTK3)	Q07002	S470	RGKNRRQSIF_____	?
PCTAIRE3 (CDK18, PCTK3)	Q07002	S87	RQNQRRFSMEDVSKR	+
PDE10A iso2	Q9Y233-2	T16	ASCFRRLTECFLSPS	?
PDE1A	P54750	S119	EEKPKFRSIVHAVQA
PDE1A	P54750	T137	VERMYRKTYHMVGLA
PDE3A	Q14432	S312	SKSHRRTSLPCIPRE
PDE3B	Q13370	S296	IRPRRRSSCVSLGET
PDE3B	Q13370	S318	CKIFRRPSLPCISRE
PDE3B	Q13370	S73	PQQPRRCSPFCRARL
PDE4A	P27815	S145	ATSQRRESFLYRSDS	+
PDE4B	Q07343	S133	GHSQRRESFLYRSDS	+
PDE4C	Q08493	S119	PHSQRRESFLYRSDS	+
PDE4D iso2	Q08499-2	S12	NNFFRRHSWICFDVD
PDE4D iso6	Q08499-6	S126	VHSQRRESFLYRSDS
PDE5A	O76074	S102	GTPTRKISASEFDRP	+
PDE6G	P18545	T35	PKFKQRQTRQFKSKP
PDIA6	Q15084	S375	KFALLKGSFSEQGIN
PDIA6	Q15084	S88	VDADKHHSLGGQYGV
PDZK1	Q5T2W1	S505	MAKERAHSTASHSSS
PEDF	P36955	S227	KFDSRKTSLEDFYLD
PEDF	P36955	T226	TKFDSRKTSLEDFYL
perilipin	O60240	S223	PSLLRRVSTLANTLS
perilipin	O60240	S277	QAVSRRRSEVRVPWL
perilipin	O60240	S497	EKPKRRVSDSFFRPS
perilipin	O60240	S522	YSQLRKKS_______
Perilipin	O60240	S81	EPVVRRLSTQFTAAN
PFKFB1	P16118	S33	RLQRRRGSSIPQFTN
PFKFB2	O60825	S466	PVRMRRNSFTPLSSS
PFKFB2	O60825	S483	IRRPRNYSVGSRPLK
PFKFB3	Q16875	S461	NPLMRRNSVTPLASP
PFKL	P17858	T773	LEHVTRRTLSMDKGF
PFKM	P08237	S774	EHITRKRSGEAAV__
PFKP	Q01813	S12	DSRAPKGSLRKFLEH
PGM5	Q15124	T31	GGGLRRPTGLFEGQR
PHKg1	Q16816	S81	VDILRKVSGHPNIIQ
PHKg1	Q16816	Y337	EIVIRDPYALRPLRR
Phosducin	P20941	S73	ERVSRKMSIQEYELI
Phospholamban	P26678	S16	RSAIRRASTIEMPQQ
Phospholamban	P26678	T17	SAIRRASTIEMPQQA
PIK3R1	P27986	S83	YIGRKKISPPTPKPR	+
Pin1	Q13526	S16	PGWEKRMSRSSGRVY	-
PIP5K1B	O14986	S214	STYKRRASRKEREKS	-
PKACa (PRKACA)	P17612	S11	AAAAKKGSEQESVKE
PKACa (PRKACA)	P17612	S140	LRRIGRFSEPHARFY
PKACa (PRKACA)	P17612	S339	EEEEIRVSINEKCGK	+
PKACa (PRKACA)	P17612	T198	RVKGRTWTLCGTPEY	+
PKAR2A	P13861	S98	SRFNRRVSVCAETYN
PKAR2A	P13861	S99	SRFNRRVSVCAETYN
PKAR2B	P31323	S114	NRFTRRASVCAEAYN
PLCB3	Q01970	S1105	LDRKRHNSISEAKMR	-
PLCG1	P19174	S1248	HGRAREGSFESRYQQ
PLCL1	Q15111	S95	GGRKKTVSFSSMPSE
PLCL1	Q15111	T246	PRFMWLKTVFEAADV
PLCL1	Q15111	T93	KCGGRKKTVSFSSMP
PLM	O00168	S83	EEGTFRSSIRRLSTR
PLM	O00168	S88	RSSIRRLSTRRR___
polycystin 1	P98161	S4169	PSRSSRGSKVSPDVP
Polycystin 1	P98161	S4252	SLQGRRSSRAPAGSS
POU2F1	P14859	S385	RRRKKRTSIETNIRV	-
PPAR-binding protein	Q15648	S18	EKLSKMSSLLERLHA
PPAR-binding protein	Q15648	T796	EASKLPSTSDDCPAI
PPP1R14A (CPI 17)	Q96A00	S130	GLRQPSPSHDGSLSP
PPP1R14A (CPI 17)	Q96A00	T38	QKRHARVTVKYDRRE	-
PPP1R16B	Q96T49	S337	SSLSRRTSSAGSRGK
PPP1R1A	Q13522	S6	__MEQDNSPRKIQFT
PPP1R1A	Q13522	S67	LKSTLAMSPRQRKKM
PPP1R1A	Q13522	T35	QIRRRRPTPATLVLT
PPP1R1A	Q13522	T75	PRQRKKMTRITPTMK	+
PPP1R1C	Q8WVI7	T34	QIRKRRPTPASLVIL
PPP1R3A	Q16821	S46	PQPSRRGSDSSEDIY
PPP1R3A	Q16821	S65	SSGTRRVSFADSFGF
PPP2R5D	Q14738	S573	KVLLRRKSELPQDVY
PPP2R5D	Q14738	S60	PSSNKRPSNSTPPPT
PPP2R5D	Q14738	S75	QLSKIKYSGGPQIVK
PPP2R5D	Q14738	S88	VKKERRQSSSRFNLS
prolactin	P01236	S207	LHCLRRDSHKIDNYL
PSEN1	P49768	S310	PEAQRRVSKNSKYNA
PSMC5	P62195	S120	RVALRNDSYTLHKIL
PTHR	Q03431	S473	VQAEIKKSWSRWTLA
PTHR	Q03431	S475	AEIKKSWSRWTLALD
PTHR	Q03431	S491	KRKARSGSSSYSYGP
PTPN12 (PTP-PEST)	Q05209	S39	FMRLRRLSTKYRTEK	-
PTPN12 (PTP-PEST)	Q05209	S435	KKLERNLSFEIKKVP
PTRF	Q6NZI2	S300	SRDKLRKSFTPDHVV
PTRF	Q6NZI2	S365	AGDLRRGSSPDVHAL
PTRF	Q6NZI2	S366	GDLRRGSSPDVHALL
PTRF	Q6NZI2	T302	DKLRKSFTPDHVVYA
Rabphilin 3A	Q9Y2J0	S237	GPPVRRASEARMSSS
Rabphilin 3A	Q9Y2J0	S272	AGLRRANSVQASRPA
Raf1	P04049	S233	VSSQHRYSTPHAFTF	+
Raf1	P04049	S259	SQRQRSTSTPNVHMV	-
Raf1	P04049	S43	FGYQRRASDDGKLTD	-
Raf1	P04049	S621	PKINRSASEPSLHRA	+
Rap1a	P62834	S180	KKKPKKKSCLLL___
Rap1b	P61224	S179	PGKARKKSSCQLL__
RARA	P10276	S369	YVRKRRPSRPHMFPK	-
RasGRF1	Q13972	S759	SRRRKLISLNIPIIT
RasGRF1	Q13972	S835	PSALSKQSSEVSMRE
RasGRF1	Q13972	S929	KEKYRRMSLASAGFP
Rb	P06400	S780	STRPPTLSPIPHIPR	-
RCN3	Q96D15	S117	QQRHIRDSVSAAWDT
Ret	P07949	S696	SSGARRPSLDSMENQ	+
RFC1	P35251	S366	SSPAKKESVSPEDSE
RFC1	P35251	T245	TKKARKDTEAGETFS
RGS10	O43665	S176	QTAAKRASRIYNT__
RGS4	P49798	S52	VVICQRVSQEEVKKW
RhoA	P61586	S188	ARRGKKKSGCLVL__
RHOK	Q15835	S21	AFIAARGSFDGSSSQ	-
RIL	P50479	S120	PTTSRRPSGTGTGPE
RIMS1	Q86UR5	S391	ARHERRHSDVALPRT
ROMK	P48048	S219	RVANLRKSLLIGSHI
ROMK	P48048	S313	ATCQVRTSYVPEEVL
ROMK	P48048	S44	RQRARLVSKDGRCNI
RPN1	P04843	S68	GSTSRATSFLLALEP
RPN1	P04843	T67	GGSTSRATSFLLALE
RPS19	P39019	S59	WFYTRAASTARHLYL
RPS19	P39019	S90	QRNGVMPSHFSRGSK
RRAD	P55042	S273	AGTRRRESLGKKAKR
RXRa	P19793	S27	TSPTGRGSMAAPSLH	-
RyR1	P21817	S2843	KKKTRKISQSAQTYD
RyR2	Q92736	S2029	TIRGRLLSLVEKVTY
RyR2	Q92736	S2806	YNRTRRISQTSQVSV
S6	P62753	S235	IAKRRRLSSLRASTS	+
S6	P62753	S236	AKRRRLSSLRASTSK	+
S6	P62753	S240	RLSSLRASTSKSESS	+
SCN2A	Q99250	S554	TYEKRFSSPHQSLLS
SCN2A	Q99250	S573	LFSPRRNSRASLFSF
SCN2A	Q99250	S610	DNDSRRDSLFVPHRH
SCN2A	Q99250	S623	RHGERRHSNVSQASR
SCN2A	Q99250	S686	EIRKRRSSSYHVSMD
SCN5A	Q14524	S525	TSMKPRSSRGSIFTF
SCN5A	Q14524	S528	KPRSSRGSIFTFRRR
SDPR	O95810	S338	ESFAEGHSEASLASA
SDPR	O95810	S51	EEAIRDNSQVNAVTV
SEC14L2	O76054	S289	VQISRGSSHQVEYEI
Separase	Q14674	S1501	TDNWRKMSFEILRGS
SERPINA1	P01009	T51	HPTFNKITPNLAEFA
SERPINA3K	P07759	S316	ELNLPKFSIASNYRL
SGK1	O00141	T369	DLINKKITPPFNPNV	+
SIK	P57059	S575	FQEGRRASDTSLTQG
SLC4A4	Q9Y6R1	S1026	KKKKKKGSLDSDNDD
Slob	Q7Z7A4-7	S54	EIALQQVSMFFRSEP
SMTNL1	A8MU46	S299	KAPERRVSAPARPRG
SNAP25	P60880	T138	GGFIRRVTNDARENE
SNAPIN	O95295	S50	HVHAVRESQVELREQ
Sorcin	P30626	T178	DSFRRRDTAQQGVVN
SOX9	P48436	S181	YQPRRRKSVKNGQAE	+
SOX9	P48436	S64	EPDLKKESEEDKFPV	?
Spinophilin	Q96SB3	S100	LSLPRASSLNENVDH
Spinophilin	Q96SB3	S94	SERGVRLSLPRASSL
SPTBN1	Q01082	T2159	NGATEQRTSSKESSP
SPTBN1	Q01082	T346	QQLQAFNTYRTVEKP
Src	P12931	S17	DASQRRRSLEPAENV	-
Src	P12931	Y419	RLIEDNEYTARQGAK	+
SREBP1	P36956	S338	IEKRYRSSINDKIIE
StAR	P49675	S195	RCAKRRGSTCVLAGM
StAR	P49675	S277	HLRKRLESHPASEAR
StAR	P49675	S56	INQVRRRSSLLGSRL
StAR	P49675	S57	NQVRRRSSLLGSRLE
STEP	P54829	S221	GLQERRGSNVSLTLD
STMN1	P16949	S16	KELEKRASGQAFELI
STMN1	P16949	S63	AAEERRKSHEAEVLK
STMN2	Q93045	S50	KQINKRASGQAFELI
STMN2	Q93045	S97	AAEERRKSQEAQVLK
STXBP5	Q5T5C0	S723	GSSSPHNSDDEQKMN
SUN2	Q9UH99	S116	GSESSRASGLVGRKA
SUN2	Q9UH99	S12	SQRLTRYSQGDDDGS
SUN2	Q9UH99	S54	SSNMKRLSPAPQLGP
SYN1	P17600	S9	NYLRRRLSDSNFMAN
SYN2	Q92777	S10	NFLRRRLSDSSFIAN
SYN3	O14994	S9	NFLRRRLSDSSFMAN
syndecan-1	P18827	S285	MKKKDEGSYSLEEPK
SYT12	Q8IV01	S97	GPPSRKGSLSIEDTF
Tau	P10636	S672	RVQSKIGSLDNITHV
Tau	P10636	S725	DTSPRHLSNVSSTGS
Tau iso3	P10636-3	S266	RVQSKIGSLDNITHV
Tau iso5 (Tau-C)	P10636-5	S214	GSRSRTPSLPTPPTR
Tau iso5 (Tau-C)	P10636-5	S231	KKVAVVRTPPKSPSS
Tau iso5 (Tau-C)	P10636-5	T217	SRTPSLPTPPTREPK
Tau iso8	P10636-8	S262	NVKSKIGSTENLKHQ
Tau iso8	P10636-8	S293	NVQSKCGSKDNIKHV
Tau iso8	P10636-8	S305	KHVPGGGSVQIVYKP
Tau iso8	P10636-8	S324	KVTSKCGSLGNIHHK
Tau iso9 (Tau-F)	P10636-9	S396	GAEIVYKSPVVSGDT
Tau iso9 (Tau-F)	P10636-9	S404	PVVSGDTSPRHLSNV
Tau iso9 (Tau-F)	P10636-9	S409	GTSPRHLSNVSSTGS
Tau iso9 (Tau-F)	P10636-9	S416	SNVSSTGSIDMVDSP
TBXA2R	P21731	S329	RLSTRPRSLSLQPQL
TBXA2R iso2	P21731-2	S330	LSTRPRRSLTLWPSL
TBXA2R iso2	P21731-2	T332	TRPRRSLTLWPSLEY
TEF-1	P28347	S102	QVLARRKSRDFHSKL	-
TGM2	P21980	S215	GRDCSRRSSPVYVGR	?
TGM2	P21980	S216	RDCSRRSSPVYVGRV	?
TH	P07101	S182	LSGVRQVSEDVRSPA
TH	P07101	S71	RFIGRRQSLIEDARK	+
Titin	Q8WZ42	S4185	IQQGAKTSLQEEMDS
TNNI3	P19429	S149	TLRRVRISADAMMQA
TNNI3	P19429	S165	LGARAKESLDLRAHL
TNNI3	P19429	S22	PAPIRRRSSNYRAYA
TNNI3	P19429	S23	APIRRRSSNYRAYAT
TNNI3	P19429	S38	EPHAKKKSKISASRK
TNNI3	P19429	S41	AKKKSKISASRKLQL
TNNI3	P19429	S43	KKSKISASRKLQLKT
TNNI3	P19429	S76	GEKGRALSTRCQPLE
TNNI3	P19429	T142	RGKFKRPTLRRVRIS
TP2	Q05952	S109	HRAKRRSSGRRYK__
TP2	Q05952	V125	MAKRIQQvYKTKTRS
TPH1	P17752	S58	RKSKRRNSEFEIFVD
TPH2	Q8IWU9	S104	RKSRRRSSEVEIFVD
TPH2	Q8IWU9	S19	YWARRGFSLDSAVPE
TPPP	O94811	S159	SGVTKAISSPTVSRL
TPPP	O94811	S32	DRAAKRLSLESEGAG
TPPP	O94811	T92	VIDGRNVTVTDVDIV
TRPV5	Q9NQA5	T708	WEILRQNTLGHLNLG
TTF-1	P43699	S336	DLAHHAASPAALQGQ
TTF-1	P43699	T9	SMSPKHTTPFSVSDI
UHRF1	Q96T88	S298	DNPMRRKSGPSCKHC
VASP	P50552	S156	EHIERRVSNAGGPPA
VASP	P50552	S238	GAKLRKVSKQEEASG
VASP	P50552	T277	LARRRKATQVGEKTP
Vax2	Q9UIW0	S170	RDLEKRASSSASEAF	?
VDR	P11473	S182	TPSFSGDSSSSCSDH	-
Vimentin	P08670	S25	PGTASRPSSSRSYVT
Vimentin	P08670	S39	TTSTRTYSLGSALRP	-
Vimentin	P08670	S412	EGEESRISLPLPNFS
Vimentin	P08670	S47	LGSALRPSTSRSLYA
Vimentin	P08670	S51	LRPSTSRSLYASSPG
Vimentin	P08670	S66	GVYATRSSAVRLRSS
Vimentin	P08670	S7	_MSTRSVSSSSYRRM
Vimentin	P08670	S72	SSAVRLRSSVPGVRL
Vimentin	P08670	S73	SAVRLRSSVPGVRLL
VR1	Q8NER1	S117	LRLYDRRSIFEAVAQ
VR1	Q8NER1	S502	YFLQRRPSMKTLFVD
VR1	Q8NER1	S6	__MEQRASLDSEESE
VR1	Q8NER1	S775	EGVKRTLSFSLRSSR
VR1	Q8NER1	S821	YLRQFSGSLKPEDAE
VR1	Q8NER1	T145	QKSKKHLTDNEFKDP
VR1	Q8NER1	T371	RHLSRKFTEWAYGPV
VTN	P04004	S397	NQNSRRPSRATWLSL
WT1	P19544	S365	KDCERRFSRSDQLKR
WT1	P19544	S393	KTCQRKFSRSDHLKT
Zyxin	Q15942	S142	PQPREKVSSIDLEID
Zyxin	Q15942	S143	QPREKVSSIDLEIDS
5-LO (ALOX5)	P09917	S272	CSLERQLSLEQEVQQ	+
MAPT/TAU	P10636	S579	NVKSKIGSTENLKHQ
MYLK1 (smMLCK)	Q15746	S1388	LATCRSTSFNVQDLL
14-3-3 eta	Q04917	S58	VVGARRSSWRVISSI
14-3-3 zeta (YWHAZ)	P63104	S58	VVGARRSSWRVVSSI
5-LO	P09917	S271	CSLERQLSLEQEVQQ
5-LO	P09917	S523	GMRGRKSSGFPKSVK	-
AA-NAT	Q16613	S205	HPFLRRNSGC_____
AA-NAT	Q16613	T31	PSCQRRHTLPASEFR
ABCA1	O95477	S1042	GGMQRKLSVALAFVG
ABCA1	O95477	S2054	GGNKRKLSTAMALIG
ABCB1	P08183	S667	SSLIRKRSTRRSVRG
ABCB1	P08183	S671	RKRSTRRSVRGSQAQ
ABCB1	P08183	S683	QAQDRKLSTKEALDE
ABCC9	O60706	S1468	RAFVRKSSILIMDEA
ABCC9	O60706	T635	FESCKKHTGVQPKTI
ACC1	Q13085	S1201	IPTLNRMSFSSNLNH
ACC1	Q13085	S78	LALHIRSSMSGLHLV
ACTA1	P68133	S201	ILTERGYSFVTTAER
ACTA1	P68133	S35	APRAVFPSIVGRPRH
ACTB	P60709	S33	APRAVFPSIVGRPRH
ACTG1	P63261	S33	APRAVFPSIVGRPRH
ACTG2	P63267	S200	ILTERGYSFVTTAER
ACTG2	P63267	S34	APRAVFPSIVGRPRH
ACTN1	P12814	S404	EKFRQKASIHEAWTD
ACTN4	O43707	S423	EKFRQKASIHEAWTD
ADD1	P35611	S408	REKSKKYSDVEVPAS
ADD1	P35611	S436	TCSPLRHSFQKQQRE
ADD1	P35611	S481	KEDGHRTSTSAVPNL
ADD1	P35611	S59	MEQKKRVSMILQSPA
ADD1	P35611	S726	KKKFRTPSFLKKSKK
ADD2	P35612	S713	KKKFRTPSFLKKSKK
ADD2	P35612	S726	KKKEKVES_______
ADD2	P35612	T55	MEQKKRVTMILQSPS
ADD3	Q9UEY8	S693	KKKFRTPSFLKKNKK
ADRB1	P08588	S312	RAGKRRPSRLVALRE
ADRB2	P07550	S262	GHGLRRSSKFCLKEH
ADRB2	P07550	S345	ELLCLRRSSLKAYGN
ADRB2	P07550	S346	LLCLRRSSLKAYGNG
AHNAK	Q09666	S135	KIKPRLKSEDGVEGD
AHNAK	Q09666	S5464	EGPKVKGSLGATGEI
AHNAK	Q09666	T158	ITVTRRVTAYTVDVT
AID	Q9GZX7	S38	YVVKRRDSATSFSLD
AID	Q9GZX7	T27	WAKGRRETYLCYVVK
AKAP13	Q12802	S1565	LSPFRRHSWGPGKNA
AKAP13	Q12802	S2733	SVSPKRNSISRTHKD
AKAP9	Q99996	S55	QKKKRKTSSSKHDVS
Aldolase A	P04075	S46	SIAKRLQSIGTENTE
AMPKa1 (PRKAA1)	Q13131	S487	ATPQRSGSVSNYRSC
ANPa R	P16066	S529	RHLRSAGSRLTLSGR	+
ANXA1	P04083	T215	AGERRKGTDVNVFNT
AP-2 alpha	P05549	S239	AEVQRRLSPPECLNA
APOBEC3G	Q9HC16	T32	PILSRRNTVWLCYEV
AQP2	P41181	S256	REVRRRQSVELHSPQ
ARFGEF1	Q9Y6D6	S883	EIAGKKISMKETKEL
ARHGDIA	P52565	S174	KGMLARGSYSIKSRF
ARHGEF7 (BETAPIX)	Q14155	S694	RKPERKPSDEEFASR	+
ARHGEF7 iso2	Q14155-2	T654	EFASRKSTAALEEDA
ARPP-21	Q86V31	S58	QERRKSKSGAGKGKL
ASIC1	P78348	S524	CQKEAKRSSADKGVA
ASIC1	P78348	S525	QKEAKRSSADKGVAL
ATF2	P15336	S62	FGPARNDSVIVADQT	+
ATP1A1	P05023	S943	ICKTRRNSVFQQGMK	-
ATP5J	P18859	S57	YKSKRQTSGGPVDAS
AurA (STK15)	O14965	T288	APSSRRTTLCGTLDY	+
AurC (STK13)	Q9UQB9	T198	TPSLRRKTMCGTLDY	+
AVPR2	P30518	S255	RRGRRTGSPGEGAHV
B-CK	P12277	S178	GRYYALKSMTEAEQQ
B-Raf	P15056	S429	PQRERKSSSSSEDRN	-
B-Raf	P15056	S446	KTLGRRDSSDDWEIP	+
Bad	Q92934	S118	GRELRRMSDEFVDSF	-
Bad	Q92934	S75	EIRSRHSSYPAGTED	-
Bad	Q92934	S99	PFRGRSRSAPPNLWA	-
BARK1 (GRK2, ADRBK1)	P25098	S685	VPLVQRGSANGL___
BCAM (Lu)	P50895	S621	SGGARGGSGGFGDEC	+
bFGF	P09038	T254	TYRSRKYTSWYVALK
BIRC5	O15392	S20	FLKDHRISTFKNWPF
BRSK2	Q8IWQ3	T260	VDAARRLTLEHIQKH	+
C-EBPb	P17676	S288	NNIAVRKSRDKAKMR
CACNA1B	Q00975	S2129	SEKQRFYSCDRFGGR
CACNA1C	Q13936	S1981	ASLGRRASFHLECLK
CACNA1D	Q01668	S121	PIRRACISIVEWKPF
CACNA1D	Q01668	S1659	PEIRRAISCDLQDDE
CACNA1D	Q01668	S1700	VNSDRRDSLQQTNTT
CACNA1D	Q01668	S1745	HNHHNHNSIGKQVPT
CACNA1D	Q01668	S1816	AAHGKRPSIGDLEHV
CACNA1D	Q01668	S1902	DSPVCYDSRRSPRRR
CACNA1D	Q01668	S1922	PASHRRSSFNFECLR
CACNA1D	Q01668	S2123	NGDVGPLSHRQDYEL
CACNA1D	Q01668	S45	GPTSQPNSSKQTVLS
CACNA1D	Q01668	S46	PTSQPNSSKQTVLSW
CACNA1D	Q01668	S497	GSLCQAISKSKLSRR
CACNA1D	Q01668	S499	LCQAISKSKLSRRWR
CACNA1D	Q01668	S52	SSKQTVLSWQAAIDA
CACNA1D	Q01668	S799	EVNQIANSDNKVTID
CACNA1D	Q01668	S864	IAPIPEGSAFFILSK
CACNA1D	Q01668	S870	GSAFFILSKTNPIRV
CACNA1D	Q01668	T1752	SIGKQVPTSTNANLN
CACNA1D	Q01668	T2105	EMESAASTLLNGNVR
CACNA1D	Q01668	T443	KGYLDWITQAEDIDP
CACNA1D	Q01668	T49	QPNSSKQTVLSWQAA
CACNA1D	Q01668	T804	ANSDNKVTIDDYREE
CACNA1H	O95180	S1107	LPDSRRGSSSSGDPP
CACNA1S	Q13698	S1617	NFLERTNSLPPVMAN
CACNA1S	Q13698	S687	EKKRRKMSKGLPDKS
CACNB2	Q08289	S514	SAPIRSASQAEEEPS
CACNB2	Q08289	S533	KKSQHRSSSSAPHHN
CACNB2	Q08289	S534	KSQHRSSSSAPHHNH
CACNG2	Q9Y698	T321	NTANRRTTPV_____
CAD	P27708	S1406	GAGGRRLSSFVTKGY	+
CAD	P27708	S1859	PPRIHRASDPGLPAE	+
Calnexin (CANX)	P27824	S583	EDEILNRSPRNRKPR
Calpain 2	P17655	S368	DGNWRRGSTAGGCRN
Calpain 2	P17655	T369	GNWRRGSTAGGCRNY
CaMKK1	Q8N5S9	S458	KSMLRKRSFGNPFEP	-
CaMKK1	Q8N5S9	S475	RREERSMSAPGNLLV	-
CaMKK1	Q8N5S9	S52	PPRARAASVIPGSTS
CaMKK1	Q8N5S9	S74	SLSARKLSLQERPAG	+
CaMKK1	Q8N5S9	T108	PRAWRRPTIESHHVA	+
Caspase 9	P55211	S183	GLRTRTGSNIDCEKL
Caspase 9	P55211	S195	EKLRRRFSSLHFMVE
Caspase 9	P55211	S99	NRQAAKLSKPTLENL
CBX3	Q13185	S93	KDGTKRKSLSDSESD
CD44	P16070	S697	AVEDRKPSGLNGEAS
Ces3	Q6UWW8	S506	ANFARNGSPNGGGLP
CETN2	P41208	S170	LRIMKKTSLY_____
CFTR	P13569	S660	FSAERRNSILTETLH
CFTR	P13569	S700	FGEKRKNSILNPINS
CFTR	P13569	S712	INSIRKFSIVQKTPL
CFTR	P13569	S737	EPLERRLSLVPDSEQ
CFTR	P13569	S753	EAILPRISVISTGPT
CFTR	P13569	S768	LQARRRQSVLNLMTH
CFTR	P13569	S795	TASTRKVSLAPQANL
CFTR	P13569	S813	DIYSRRLSQETGLEI
CGb	P01233	T117	CALCRRSTTDCGGPK
CHCHD3	Q9NX63	T11	TTSTRRVTFEADENE
CIITA	P33076	S1050	AASLLRLSLYNNCIC
CIITA	P33076	S834	QELPGRLSFLGTRLT
CK1d (CSNK1D)	P48730	S370	MERERKVSMRLHRGA	-
Claudin 3	O15551	T192	PPREKKYTATKVVYS
COL4A3	Q01955	S1435	LKGKRGDSGSPATWT
COL4A3	Q01955	S1452	GFVFTRHSQTTAIPS
Connexin 32	P08034	S233	NPPSRKGSGFGHRLS
Coronin 1C	Q9ULV4	S354	IMTVPRKSDLFQDDL
CP	P00450	T688	SKGERRDTANLFPHK
CREB1	P16220	S133	EILSRRPSYRKILND	+
CREM	Q03060-2	S71	EILSRRPSYRKILNE
CRF-R1	P34998	S330	LMTKLRASTTSETIQ
CSF2RB	P32927	S601	LGPPHSRSLPDILGQ
CSK	P41240	S364	ALREKKFSTKSDVWS	+
CSP	Q9H3Z4	S10	DQRQRSLSTSGESLY	-
CTNNB1	P35222	S45	GATTTAPSLSGKGNP	+
CTNNB1	P35222	S552	QDTQRRTSMGGTQQQ
CTNNB1	P35222	S675	QDYKKRLSVELTSSL
CTPS	P17812	T455	MRLGKRRTLFQTKNS
CUX1 (CUTL1)	P39880	S1215	AYMKRRHSSVSDSQP	-
CUX1 (CUTL1)	P39880	S1237	TEYSQGASPQPQHQL	-
CUX1 (CUTL1)	P39880	S1270	YQQKPYPSPKTIEDL	-
Cx36	Q9UKL4	S110	KQRERRYSTVFLALD
Cx36	Q9UKL4	S293	GAQAKRKSIYEIRNK
Cx36	Q9UKL4	S306	NKDLPRVSVPNFGRT
Cx36	Q9UKL4	S315	PNFGRTQSSDSAYV_
Cx43	P17302	S364	IVDQRPSSRASSRAS
Cx43	P17302	S367	QRPSSRASSRASSRP
Cx43	P17302	S368	RPSSRASSRASSRPR
Cx43	P17302	S372	RASSRASSRPRPDDL
Cyclin D1 (CCND1)	P24385	S197	ATDVKFISNPPSMVA
Cyclin D1 (CCND1)	P24385	S234	YRLTRFLSRVIKCDP
Cyclin D1 (CCND1)	P24385	S90	NYLDRFLSLEPVKKS
CYP3A5	P20815	S392	GVFIPKGSMVVIPTY
DAP3	P51398	S185	FDQPLEASTWLKNFK
DAP3	P51398	S220	RESTEKGSPLGEVVE
DAP3	P51398	S31	MGTQARQSIAAHLDN
DAP3	P51398	T186	DQPLEASTWLKNFKT
DARPP-32	Q9UD71	S137	EEEEEEDSQAEVLKV
DARPP-32	Q9UD71	T34	MIRRRRPTPAMLFRL
Dematin	Q08495	S403	NELKKKASLF_____
Desmin	P17661	S11	YSSSQRVSSYRRTFG

Protein Kinase Specificity

Matrix of observed frequency (%) of amino acids in aligned protein substrate phosphosites

Matrix Type:

Experimentally derived from alignment of 1115 known protein substrate phosphosites.

Domain #:

Inhibitors

For further details on these inhibitors click on the Compound Name and enter it into DrugKiNET or click on the ID's

Based on in vitro and/or in vivo phosphorylation data

Compound Name	KD, Ki or IC50 (nM)	PubChem ID	ChEMBL ID	PubMed ID

CHEMBL213618	IC50 = 100 pM	16043304	213618	16765046
Staurosporine	IC50 = 900 pM	5279		16765046
AC1OCAB6	IC50 = 2.1 nM	6914611	383264	16413780
AT13148	IC50 = 3 nM
Ophiocordin	Ki = 4.7 nM	5287736	60254	16134928
A 443654	IC50 < 6 nM	10172943	379300
TTT-3002	IC50 < 6 nM
CHEMBL1080569	IC50 = 8 nM	11568147	1080569	20137932
Isoquinoline-pyridine; 10y	IC50 = 8 nM	15604547		16603355
A674563	Ki = 16 nM	11314340	379218	16678413
Aminopurvalanol A	IC50 = 18 nM	6604931		12678910
SCH 47112	IC50 = 18.5 nM	9932061
GSK690693	Kd = 19 nM	16725726	494089	22037378
AT7867	IC50 = 20 nM	11175137	428462	20423992
CHEMBL493169	Ki > 21 nM	44219632	493169	19414255
7-hydroxystaurosporine	IC50 > 30 nM	72271	1236539
Balanol analog 5	IC50 = 30 nM	5327922	52529	9003521
SureCN2505235	IC50 = 33 nM	23649240		22934575
CHEMBL383541	IC50 = 38 nM	6914613	383541	16403626
CHEMBL554986	Kd = 48 nM	11250806	554986	15634010
Balanol analog 4	IC50 = 70 nM	5327921		9003521
AT9283	IC50 > 100 nM	24905142		19143567
Gö7874	IC50 = 100 nM	5327863
H-89	IC50 < 100 nM	449241	104264
Ro-31-8220	IC50 = 100 nM	5083	6291
Ruboxistaurin	IC50 = 100 nM	153999	91829
Staurosporine aglycone	IC50 = 100 nM	3035817	281948
SureCN2579964	IC50 < 100 nM	290221	233000	22934575
CHIR-124	IC50 = 103 nM	11502647		17255282
BX912	IC50 = 110 nM	11754511		15772071
SureCN2934634	IC50 = 130 nM	45375865	1080994	19854645
CHEMBL534916	Kd = 150 nM	11490873	534916	15634010
K-252a; Nocardiopsis sp.	IC50 > 150 nM	3813	281948	22037377
CCT128930	IC50 < 168 nM	17751819		18345609
Meridianin B	IC50 = 210 nM	10380356		15026054
Lestaurtinib	Kd = 220 nM	126565		22037378
N-Benzoylstaurosporine	IC50 > 250 nM	56603681	608533	22037377
PKR Inhibitor	IC50 > 250 nM	6490494	235641	22037377
BMS-690514	IC50 = 280 nM	11349170		21531814
Gö6976	IC50 > 300 nM	3501	302449
Enzastaurin	Kd = 330 nM	176167	300138	22037378
CHEMBL1258913	IC50 = 332 nM	11847343	1258913	20817473
GSK269962A	IC50 < 400 nM	16095342	220241
CHEMBL1171647	IC50 = 450 nM	25061501	1171647	20481595
Fasudil	Ki = 460 nM	3547	38380	16249185
CHEMBL606245	IC50 = 490 nM	44160269	606245	19884013
CHEMBL350335	IC50 = 500 nM	9847089	350335
PP1 Analog II; 1NM-PP1	IC50 = 500 nM	5154691	573578	22037377
SB218078	IC50 = 500 nM	447446	289422	22037377
BMS-777607	IC50 = 550 nM	24794418		19260711
Amgen TBK 1 inhibitor (Compound II)	IC50 < 600 nM
Ponatinib	IC50 = 613 nM	24826799		20513156
Meridianin C	IC50 = 700 nM	10017019	44541	15026054
H-1152; Glycyl	IC50 < 800 nM	16760635
PP1 Analog II; 1NM-PP1	IC50 < 800 nM	5154691	573578
SB747651A	IC50 < 800 nM	11393719	188434
BX795	IC50 = 840 nM	10077147	577784	15772071
BX517	IC50 > 900 nM	11161844	228654
Alisertib	IC50 > 1 µM	24771867		22016509
Baricitinib	IC50 > 1 µM	44205240		20363976
CK7	Ki > 1 µM	447961		15027857
CP673451	IC50 > 1 µM	10158940		15705896
KIN 112	IC50 = 1 µM
Kinome_714	IC50 > 1 µM	46886323		20346655
KT5720	IC50 = 1 µM	3844	608532
MK5108	IC50 > 1 µM	24748204		20053775
PP1	IC50 = 1 µM	1400	306380
PP242	IC50 = 1 µM	25243800
R406	IC50 = 1 µM	11984591
Rottlerin	IC50 < 1 µM	5281847	34241
Ruxolitinib	IC50 = 1 µM	25126798	1789941
SGI-1776	IC50 > 1 µM			19734450
Silmitasertib	IC50 > 1 µM	24748573		21174434
SNS314	IC50 > 1 µM	16047143	514582	18678489
Tozasertib	IC50 = 1 µM	5494449	572878
WZ3146	Kd > 1 µM	44607360		20033049
WZ4002	Kd > 1 µM	44607530		20033049
CHEMBL338049	IC50 = 1.15 µM	44352972	338049
Ro 31-8425	IC50 = 1.2 µM		421565	8421286
SureCN2934911	IC50 = 1.3 µM	45375866	1080995	19854645
BX320	IC50 = 1.4 µM	657138	573108	15772071
BMS-754807	IC50 = 1.605 µM	24785538		19996272
K-252c	IC50 = 2 µM	3815	16958	14552791
Momelotinib	IC50 > 2 µM	25062766		19295546
CHEMBL536485	IC50 = 2.4 µM	11445419	536485	15634010
PP121	IC50 < 2.5 µM	24905142		18849971
Ro-32-0557	IC50 = 2.8 µM	19095896
CHEMBL306856	IC50 = 2.81 µM	5330769	306856	12824014
CHEMBL307152	IC50 = 2.93 µM	11655119	307152	12824014
CHEMBL75368	IC50 = 2.99 µM	5330777	75368	12824014
Doramapimod	IC50 > 3 µM	156422	103667
Kenpaullone	IC50 > 3 µM	3820	296586
TG101209	IC50 > 3 µM	16722832		17541402
GDC-0068	IC50 = 3.1 µM	24995523		22934575
CHEMBL73781	IC50 = 3.15 µM	5330771	73781	12824014
CHEMBL306501	IC50 = 3.38 µM	5330768	306501	12824014
AST-487	Kd = 3.7 µM	11409972	574738	18183025
CHEMBL73718	IC50 = 3.74 µM	5330766	73718	12824014
Purvalanol B	IC50 = 3.8 µM	448991	23254	12036347
CHEMBL494221	Ki > 4 µM	44219633	494221	19414255
JNJ-28871063	IC50 > 4 µM	17747413		17975007
NVP-TAE684	Kd = 4 µM	16038120	509032	22037378
Pyrimidylpyrrole, 11e	Ki > 4 µM	11634725	583042	19827834
Bisindolylmaleimide VII	IC50 = 4.4 µM	369882
1;9-Pyrazoloanthrone	IC50 > 4.5 µM	8515	7064
Alsterpaullone	IC50 > 4.5 µM	5005498	50894
HG-9-91-01	IC50 > 4.5 µM
Rapamycin	IC50 > 4.5 µM	5284616	413
CHEMBL308979	IC50 = 4.58 µM	5330778	308979	12824014

Disease Linkage

General Disease Association:

Cancer, endocrine disorders

Specific Diseases (Non-cancerous):

Primary pigmented nodular adrenocortical disease (PPNAD); Cushing syndrome, ACTH-independent adrenal, somatic

Specific Cancer Types:

Hepatocellular fibrolamellar carcinomas

Comments:

PKACa may be an oncoprotein (OP), although this kinase has also been implicated in inhibiting normal cell proliferation. Gain-of-function mutations in the PRKACA catalytic domain are associated with elevated catalytic activity of the kinase, and have been observed in several cancer types. In particular, a L206R subsitution mutation is observed in cancer and in Cushing's syndrome patients. The gain-of-function mutation is predicted to result in the interruption of the interaction between the catalytic and regulatory subunits of protein kinase A, resulting in a constitutively active PKA. Gain-of-function mutations in the PRKACA gene that lead to consitutive activity of the PKA holoenzyme have been suggested to play an important role in the development of several types of cancer.

Gene Expression in Cancers:

TranscriptoNET (www.transcriptonet.ca) analysis with mRNA expression data retrieved from the National Center for Biotechnology Information's Gene Expression Omnibus (GEO) database, which was normalized against 60 abundantly and commonly found proteins, indicated altered expression for this protein kinase as shown here as the percent change from normal tissue controls (%CFC) as supported with the Student T-test in the following types of human cancers: Brain glioblastomas (%CFC= +880, p<(0.0003); Brain oligodendrogliomas (%CFC= +968, p<0.076); and Uterine leiomyomas (%CFC= +182, p<0.003). The COSMIC website notes an up-regulated expression score for PKACa in diverse human cancers of 466, which is close to the average score of 462 for the human protein kinases. The down-regulated expression score of 91 for this protein kinase in human cancers was 1.5-fold of the average score of 60 for the human protein kinases.

Mutagenesis Experiments:

Insertional mutagenesis studies in mice have not yet revealed a role for this protein kinase in mouse cancer oncogenesis.

Mutation Rate in All Cancers:

Percent mutation rates per 100 amino acids length in human cancers: 0.09 % in 25371 diverse cancer specimens. This rate is only 18 % higher than the average rate of 0.075 % calculated for human protein kinases in general.

Mutation Rate in Specific Cancers:

Highest percent mutation rates per 100 amino acids length in human cancers: 0.39 % in 1093 large intestine cancers tested.

Frequency of Mutated Sites:

Most frequent mutations with the number of reports indicated in brackets: L206R (4).

Comments:

Only 2 deletions and 1 insertion, and no complex mutations are noted on the COSMIC website.

COSMIC Entry:

PRKACA

OMIM Entry:

601639

Nomenclature

Short Name:

Full Name:

Alias:

Classification

Type:

Group:

Family:

SubFamily:

Specific Links

General Links

Structure

Mol. Mass (Da):

# Amino Acids:

# mRNA Isoforms:

mRNA Isoforms:

4D Structure:

3D Structure:

1D Structure:

3D Image (rendered using PV Viewer):

PDB ID

Subfamily Alignment

Domain Distribution:

Kinexus Products

hiddentext

Post-translation Modifications

Acetylated:

Myristoylated:

Serine phosphorylated:

Threonine phosphorylated:

Tyrosine phosphorylated:

Ubiquitinated:

Distribution

Human Tissue Distribution

% Max Expression: Mean Expression: Number of Samples: Standard Deviation:

% Max Expression: Mean Expression: Number of Samples: Standard Deviation:

Evolution

Species Conservation

PhosphoNET % Identity: PhosphoNET % Similarity: Homologene %Identity:

PhosphoNET % Identity: PhosphoNET % Similarity: Homologene %Identity:

Regulation

Activation:

Inhibition:

Synthesis:

Degradation:

Known Upstream Kinases

Based on in vitro and/or in vivo phosphorylation data

Known Downstream Substrates

Based on in vitro and/or in vivo phosphorylation data

Protein Kinase Specificity

Matrix Type:

Domain #:

Inhibitors

Based on in vitro and/or in vivo phosphorylation data

Disease Linkage

General Disease Association:

Specific Diseases (Non-cancerous):

Specific Cancer Types:

Comments:

Gene Expression in Cancers:

Mutagenesis Experiments:

Mutation Rate in All Cancers:

Mutation Rate in Specific Cancers:

Frequency of Mutated Sites:

Comments:

COSMIC Entry:

OMIM Entry:

% Max Expression:

Mean Expression:

Number of Samples:

Standard Deviation:

% Max Expression:

Mean Expression:

Number of Samples:

Standard Deviation:

PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity:

PhosphoNET % Identity:
PhosphoNET % Similarity:
Homologene %
Identity: